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A Reverse Engineering Approach for Imaging Neuronal Architecture – Large-Area, High-Resolution SEM Imaging
2016
Microscopy Today
A reverse engineering approach to scanning electron microscope (SEM) imaging of neuronal architecture provides largearea maps of a sample, which links the function of a cell with its location in a tissue. The Chipscanner's laser interferometer stage and field-of-view mapping allow high-resolution SEM images to be stitched together. This workflow produces accurate, high-resolution maps of tissue over biologically relevant length scales in reasonable time frames. A 2.5 × 1.8 mm mouse spinal cord
doi:10.1017/s1551929516000730
fatcat:2imajajylrdajii5hmymugffhy