A Reverse Engineering Approach for Imaging Neuronal Architecture – Large-Area, High-Resolution SEM Imaging

Christine A. Brantner, Martin Rasche, Kevin E. Burcham, Joseph Klingfus, Joel Fridmann, Jason E. Sanabia, Can E. Korman, Anastas Popratiloff
2016 Microscopy Today  
A reverse engineering approach to scanning electron microscope (SEM) imaging of neuronal architecture provides largearea maps of a sample, which links the function of a cell with its location in a tissue. The Chipscanner's laser interferometer stage and field-of-view mapping allow high-resolution SEM images to be stitched together. This workflow produces accurate, high-resolution maps of tissue over biologically relevant length scales in reasonable time frames. A 2.5 × 1.8 mm mouse spinal cord
more » ... esin section was imaged in less than 24 hours. This is the most accurate, large-area map of neuronal tissue directly acquired by an SEM.
doi:10.1017/s1551929516000730 fatcat:2imajajylrdajii5hmymugffhy