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In this study, the optical properties of glycated (HbA1c) and nonglycated (Hb) hemoglobin are compared using Surface-enhanced Raman spectroscopy (SERS), spectrofluorimetry, and refractometry. Analysis of the spectral shift of SERS spectra showed good discrimination between two hemoglobins indicating differences in their molecular structure. The fluorescence spectra measured at excitation wavelengths of 260, 270, and 280 nm also indicate differences in the molecular structure of thesedoi:10.18287/jbpe22.08.020303 fatcat:a4hglf6d5vehpjf46kgzpnsgdy