Review of bio-optical imaging systems with a high space-bandwidth product

Jongchan Park, David J. Brady, Guoan Zheng, Lei Tian, Liang Gao
2021 Advanced Photonics  
Optical imaging has served as a primary method to collect information about biosystems across scales-from functionalities of tissues to morphological structures of cells and even at biomolecular levels. However, to adequately characterize a complex biosystem, an imaging system with a number of resolvable points, referred to as a space-bandwidth product (SBP), in excess of one billion is typically needed. Since a gigapixel-scale far exceeds the capacity of current optical imagers, compromises
more » ... t be made to obtain either a low spatial resolution or a narrow field-of-view (FOV). The problem originates from constituent refractive optics-the larger the aperture, the more challenging the correction of lens aberrations. Therefore, it is impractical for a conventional optical imaging system to achieve an SBP over hundreds of millions. To address this unmet need, a variety of high-SBP imagers have emerged over the past decade, enabling an unprecedented resolution and FOV beyond the limit of conventional optics. We provide a comprehensive survey of high-SBP imaging techniques, exploring their underlying principles and applications in bioimaging.
doi:10.1117/1.ap.3.4.044001 pmid:35178513 pmcid:PMC8849623 fatcat:vfjbb2ru7zaytf4s5epzcym6nm