Primo, F. L. Photodynamic Process for tissue biostimulation on in vitro human skin model using low-intensity laser and chloroaluminium phthalocyanine nanoemulsion. 2009. 169f. Thesis (Doctoral). Faculdade de Ciências Farmacêuticas de Ribeirão Preto -Universidade de São Paulo, Ribeirão Preto, 2009. This work of doctorate thesis bases on a scientific development thoroughly multidiscipline combining protocols, techniques and experimental assays of the pharmaceutical technology, nanotechnology,

more »

... ue engineering and photobiology. The work was accomplished in three subsequent stages. Firstly were carried out studies and characterization of a drug delivery system with nanotechnology, which was evaluated thoroughly starting from physical-chemical tests and assays of cytotoxicity in human fibroblast culture. In the sequence were carried photobiological studies combining the application of low-intensity laser and nanosized photosensitizers with tissue models, for evaluation of the biological photostimulation. In mimetic conditions it was developed an in vitro three-dimensional human skin model, as tool to evaluate the photobiological response after the induction of the photodynamic processes and also as alternative for in vitro assays of drug permeation/retention. Results were promising, aiding in the understanding of interaction of extracellular matrix with photodynamic processes. Therefore, the work was based in combination of techniques with application in dermatology, pharmaceutical process, cosmetics and correlate areas. . CASTANO, A. P.; DAI, T. H.; YAROSLAVSKY, I.; COHEN, R.; APRUZZESE, W. A.; SMOTRICH, M. H.; HAMBLIN, M. R. Low-level laser therapy for zymosan-induced arthritis in rats: Importance of illumination time, Lasers in Surgery and Medicine, v. 39, p. 543-550, July2007. CASTANO, A. P.; DEMIDOVA, T. N.; HAMBLIN, M. R. Mechanisms in photodynamic therapy: part one-photosensitizers, photochemistry and cellular localization, Photodiagnosis and Photodynamic Therapy, v. 1, p. 279-293, 2004. CATORZE, M. G. Laser: fundamentos e indicações em dermatologia, Medicina Cutánea Ibero-Latino-Americana, v. 37, p. 5-27, 2009. CHEN, Y.; LIU, H. R.; ZHANG, Z. C.; WANG, S. J. Preparation of polymeric nanocapsules by radiation induced miniemulsion polymerization, European Polymer Journal, v. 43, p. 2848-2855, July2007. CLARK, R. A. F. Cutaneous Tissue-Repair -Basic Biologic Considerations .1, Journal of the American Academy of Dermatology, v. 13, p. 701-725, 1985. COLIC, M. M.; VIDOJKOVIC, N.; JOVANOVIC, M.; LAZOVIC, G. The use of polarized light in aesthetic surgery, Aesthetic Plastic Surgery, v. 28, p. 324-327, Sept.2004. COULOMB, B.; DUBERTRET, L.; BELL, E.; MERRILL, C.; FOSSE, M.; BRETON-GORIUS, J.; PROST, C.; TOURAINE, R. Endogenous peroxidases in normal human dermis: a marker of fibroblast differentiation, Journal of Investigative Dermatology, v. 81, p. 75-78, July1983a. COULOMB, B.; DUBERTRET, L.; MERRILL, C.; TOURAINE, R.; BELL, E. The Collagen Lattice -A Physiological Model for Studying Fibroblasts, Journal of Investigative Dermatology, v. 80, p. 324, 1983b. Fer 49 Tese de Doutorado Referências Bibliográficos ___________________________________________________________________________ ___________________________________________________________________________ _________________________________ COUSSENS, L. M.; FINGLETON, B.; MATRISIAN, L. M. Cancer therapy -Matrix metalloproteinase inhibitors and cancer: Trials and tribulations, Science, v. 295, p. 2387-2392, Mar.2002. COUVREUR, P.; DUBERNET, C.; PUISIEUX, F. Controlled Drug Delivery with Nanoparticles: Current Possibilities and Trends Future, European Journal of Pharmaceutical and Biopharmaceutics, v. 41, p. 2-13, July1994. COUVREUR, P.; KANTE, B.; ROLAND, M.; GUIOT, P.; BAUDUIN, P.; SPEISER, P. Polycyanoacrylate Nanocapsules As Potential Lysosomotropic Carriers -Preparation, Morphological and Sorptive Properties, Journal of Pharmacy and Pharmacology, v. 31, p. 331-332, 1979a. COUVREUR, P.; KANTE, B.; ROLAND, M.; SPEISER, P. Adsorption of Anti-Neoplastic Drugs to Polyalkylcyanoacrylate Nanoparticles and Their Release in Calf Serum, Journal of Pharmaceutical Sciences, v. 68, p. 1521-1524, 1979b. DADRAS, S.; MOHAJERANI, E.; EFTEKHAR, F.; HOSSEINI, M. Different photoresponses of Staphylococcus aureus and Pseudomonas aeruginosa to 514, 532, and 633 nm low level lasers in vitro, Current Microbiology, v. 53, p. 282-286, Oct.2006. DARWENT, J. R. Metal phthalocyanines and porphyrins as photosensitizers for reduction of water to hidrogen, Coordination Chemistry Reviews, v. 44, p. 83-126, 1982. DE PAOLI, V. M.; DE PAOLI, S. H.; BORISSEVITCH, L. E.; TEDESCO, A. C. Fluorescence lifetime and quantum yield of TMPyPH2 associated with micelles and DNA, Journal of Alloys and Compounds, v. 344, p. 27-31, Oct.2002. DE ROSA, F. S.; TEDESCO, A. C.; LOPEZ, R. F. V.; PIERRE, M. B. R.; LANGE, N.; MARCHETTI, J. M.; ROTTA, J. C. G.; BENTLEY, M. V. L. B. In vitro skin permeation and retention of 5-aminolevulinic acid ester derivatives for photodynamic therapy, Journal of Controlled Release, v. 89, p. 261-269, Apr.2003. Fer 50 Tese de Doutorado Referências Bibliográficos ___________________________________________________________________________ ___________________________________________________________________________ _________________________________ DE ROSA, M. C.; CRUTCHLEY, R. J. Photosensitized singlet oxygen and its applications, Coordination Chemistry Reviews, v. 233-234, p. 351-371, 2002. DELAEY, E.; VAN LAAR, F.; DE VOS, D.; KAMUHABWA, A.; JACOBS, P.; DE WITTE, P. A comparative study of the photosensitizing characteristics of some cyanine dyes, Journal of Photochemistry and Photobiology B: Biology, v. 55, p. 27-36, 2000. DEMAS, J. N.; CROSBY, G. A. Measurement of photoluminescence quantum yields, Journal of Physical Chemistry, v. 75, p. 991-1024, 1971. DESMOULIERE, A.; REDARD, M.; DARBY, I.; GABBIANI, G. Apoptosis mediates the decrease in cellularity during the transition between granulation tissue and scar, WHITE Effects of increased concentrations of prostaglandin E levels with epidermolysis bullosa dystrophica recessive fibroblasts within a populated collagen lattice, Journal of Investigative Dermatology, v. 81, p. 572-575, 1983. Fer 51 Tese de Doutorado Referências Bibliográficos ___________________________________________________________________________ ___________________________________________________________________________ _________________________________ FERNANDEZ, J. M.; BILGIN, M. D.; GROSSWEINER, L. I. Singlet oxygen generation by photodynamic agents, Journal of Photochemistry and Photobiology B: Biology, v. 37, p. 131-140, 1997. FERREIRA, M. C.; GAMEIRO, J.; NAGIB, P. R. A.; BRITO, V. N.; VASCONCELLOS, E. D. C.; VERINAUD, L. Effect of Low Intensity Helium-Neon (HeNe) Laser Irradiation on Experimental Paracoccidioidomycotic Wound Healing Dynamics, Fer 52 Tese de Doutorado Referências Bibliográficos ___________________________________________________________________________ ___________________________________________________________________________ _________________________________ GOMES, A. J. Obtenção, caracterização e avalaliação de micropartículas de PLGA contendo indocianina e bacterioclorofila-a como sistemas de liberação de fármacos na terapia fotodinâmica. 103 f. Tese Doutorado -FFCLRP -USP, Riberão Preto, 2003. GUINEBRETIERE, S.; BRIANCON, S.; FESSI, H.; TEODORESCU, V. S.; BLANCHIN, M. G. Nanocapsules of biodegradable polymers: preparation and characterization by direct high resolution electron microscopy, Materials Science & Tese de Doutorado Referências Bibliográficos ___________________________________________________________________________ ___________________________________________________________________________ _________________________________ IDOWU, M.; NYOKONG, T. Photophysical and photochemical properties of tetrasulfonated silicon and germanium phthalocyanines in aqueous and non-aqueous media, Journal of Photochemistry and Photobiology A: Chemistry, v. 197, p. 273-280, 2008. JAYASREE, R. S.; GUPTA, A. K.; RATHINAM, K.; MOHANAN, P. V.; MOHANTY, M.