Processos fotodinâmicos para bioestimulação tecidual em modelo in vitro de pele humana empregando-se laser de baixa potência e cloro alumínio ftalocianina em nanoemulsão [thesis]

Fernando Lucas Primo
Primo, F. L. Photodynamic Process for tissue biostimulation on in vitro human skin model using low-intensity laser and chloroaluminium phthalocyanine nanoemulsion. 2009. 169f. Thesis (Doctoral). Faculdade de Ciências Farmacêuticas de Ribeirão Preto -Universidade de São Paulo, Ribeirão Preto, 2009. This work of doctorate thesis bases on a scientific development thoroughly multidiscipline combining protocols, techniques and experimental assays of the pharmaceutical technology, nanotechnology,
more » ... ue engineering and photobiology. The work was accomplished in three subsequent stages. Firstly were carried out studies and characterization of a drug delivery system with nanotechnology, which was evaluated thoroughly starting from physical-chemical tests and assays of cytotoxicity in human fibroblast culture. In the sequence were carried photobiological studies combining the application of low-intensity laser and nanosized photosensitizers with tissue models, for evaluation of the biological photostimulation. In mimetic conditions it was developed an in vitro three-dimensional human skin model, as tool to evaluate the photobiological response after the induction of the photodynamic processes and also as alternative for in vitro assays of drug permeation/retention. Results were promising, aiding in the understanding of interaction of extracellular matrix with photodynamic processes. 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doi:10.11606/t.60.2009.tde-11122009-170544 fatcat:6ee74lu34rekni6r7uypyxkvfe