Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins

Gabriel Hancu, Hajnal Kelemen, Aura Rusu, Árpád Gyéresi
2013 Journal of the Serbian Chemical Society  
A rapid and simple capillary electrophoresis method has been developed for the simultaneous determination of six extensively used cephalosporin antibiotics (cefaclor, cefadroxil, cefalexin, cefuroxim, ceftazidim and ceftriaxon). The determination of cephalosporins was performed at pH 6.8, using a 25 mM phosphate/25 mM borate mixed buffer, and a voltage of 25 kV at a temperature of 25 °C. A baseline separation was achieved in approximately 10 min. The separation resolution was increased by the
more » ... dition of an anionic surfactant, 50 mM sodium dodecyl sulfate, to the buffer solution. The proposed separation was evaluated based on the detection and quantification limits, effective electrophoretic mobility and relative standard deviation for the migration times and peak areas.
doi:10.2298/jsc121117028h fatcat:blu2xpblb5holbfcyx4qwhru7u