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A Pre-Steady State Analysis of Ligand Binding to Human Glucokinase: Evidence for a Preexisting Equilibrium
Cooperativity with glucose is a key feature of human glucokinase (GK), allowing its crucial role as a glucose sensor in hepatic and pancreatic cells. We studied the changes in enzyme intrinsic tryptophan fluorescence induced by binding of different ligands to this monomeric enzyme using stoppedflow and equilibrium binding methods. Glucose binding data under pre-steady state conditions suggest that the free enzyme in solution is in a preexisting equilibrium between at least two conformersdoi:10.1021/bi0617308 pmid:17260972 fatcat:nuhhmzs4cza7dpsxcgaujxjjje