A study was conducted to determine the surface contaminant fungi associated with different samples of Egyptian Ras Cheese collected from different locations in Assiut City, Egypt. 52 fungal isolates assigned to six species belonging to the genera Aspergillus, Penicillium and Mucor were recovered and identified. Aspergillus was the most predominant and represented by four species namely A. flavus, A. niger, A. ustus, and A. fumigatus. Penicillium aurantiogriseum and Mucor racemosus were detected

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... as a single representative of the genera Penicillium and Mucor. All the isolated fungal species produced casienase and lipase but did not produce lactase. Analysis of the studied Ras cheese samples for the presence of aflatoxin indicated that 66.6 % of the analyzed samples were contaminated with aflatoxins B 1 , B 2 and G 2 .