Metabolic footprinting as a tool for discriminating between brewing yeasts

Georgina A. Pope, Donald A. MacKenzie, Marianne Defernez, Miguel A. M. M. Aroso, Linda J. Fuller, Fred A. Mellon, Warwick B. Dunn, Marie Brown, Royston Goodacre, Douglas B. Kell, Marcus E. Marvin, Edward J Louis (+1 others)
2007 Yeast  
The characterization of industrial yeast strains by examining their metabolic footprints (exometabolomes) was investigated and compared to genome-based discriminatory methods. A group of nine industrial brewing yeasts was studied by comparing their metabolic footprints, genetic fingerprints and comparative genomic hybridization profiles. Metabolic footprinting was carried out by both direct injection mass spectrometry (DIMS) and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS),
more » ... th data analysed by principal components analysis (PCA) and canonical variates analysis (CVA). The genomic profiles of the nine yeasts were compared by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis, genetic fingerprinting using amplified fragment length polymorphism (AFLP) analysis and microarray comparative genome hybridizations (CGH). Metabolomic and genomic analysis comparison of the nine brewing yeasts identified metabolomics as a powerful tool in separating genotypically and phenotypically similar strains. For some strains discrimination not achieved genomically was observed metabolomically. Figure 3. Metabolic footprinting of brewing yeast strains by DIMS, (a) analysed by PCA and (b) validated by subsequent semi-supervised CVA. NCYC 505 is the type strain of S. cerevisiae, NCYC 1187 and NCYC 1332 are S. cerevisiae ale strains, while all others are lager strains of S. cerevisiae, S. pastorianus or S. bayanus
doi:10.1002/yea.1499 pmid:17534862 fatcat:wv5em3xpfreujb4impkppiotqq