The Filamentous FungusAspergillus nigerContains Two "Differentially Regulated" Trehalose-6-phosphate Synthase-encoding Genes,tpsAandtpsB

Markus F. Wolschek, Christian P. Kubicek
1997 Journal of Biological Chemistry  
Two genes encoding trehalose-6-phosphate synthase were cloned from Aspergillus niger. tpsA was cloned using the Saccharomyces cerevisiae GGS1/TPS1 gene as a probe. It encodes a 517-amino acid polypeptide with 64 -70% similarity to trehalose-6-phosphate synthase of S. cerevisiae, Kluyveromyces lactis, and Schizosaccharomyces pombe. Its transcription occurs constitutively and is enhanced on carbon-derepressing carbon sources, coinciding with the presence of a CreA-binding nucleotide motif in the
more » ... -noncoding region of tpsA. Disruption of tpsA only weakly reduces growth on glucose, and neither influences the glucose induction of a low affinity glucose permease nor interferes with the catabolite repression of a pectinase; it causes reduced the heat tolerance of conidia. tpsB was cloned by a polymerase chain reaction-based strategy. Its 480 amino acid sequence showed 76.5% identity to tpsA. Its transcription was hardly detectable at ambient temperatures but was enhanced strongly upon heat shock, which agrees with the presence of several copies of a C 4 T stress-responsive element in its 5-upstream sequences. Hence the function of yeast GGS1/TPS1 has been split into two differentially regulated genes in A. niger, of which none appears to be involved in glucose sensing.
doi:10.1074/jbc.272.5.2729 pmid:9006911 fatcat:zqti3lewp5f3php54ya5m2zlc4