Use of fresh human amniotic membrane for improvement of rabbit perianal surgery wounds

Alireza Khazaei, Abdolreza Sotoodeh Jahromi, Hadi Okati, Masoum Khoshfetrat, Fahimeh Shibak
2017 Biomedical Research and Therapy  
The use of fresh human amniotic membrane as an accelerating factor for injury repair has been considered for improvement of various injuries, particularly burns, and has shown significant effects. Perianal surgeries, however, have a high recurrence rate due to the anatomy of the area. Thus, finding an effective method in postoperative care may play an important role in improving the quality of surgical procedures in this area. Considering the high recurrent rate of injury in the perianal region
more » ... and the need for more effective post-surgical care, this study was aimed at evaluating the effects of using amniotic membrane for wound improvement following perianal surgery in a rabbit model. Methods: Twenty-four New Zealand white male rabbits were divided into two groups. After the rabbits received anesthesia and perianal surgery, group A rabbits received fresh human amniotic membranes (measuring 1x1 cm) to potentially repair the surgical wounds, while group B rabbits did not receive the membranes. The data were analyzed using t-tests and in order to confirm the normal distribution of data, Kolmogorov-Simonov test was used (P=0.03). Results: In this study, the Abramov surgical wound scoring system was used to determine the wound improvement rate in all specimens. According to the results, this rate was significantly higher in the group administered with amniotic membrane (group A; P=0.0001). Conclusion: The use of fresh human amniotic membrane plays an important role in the improvement of perianal surgical wounds in rabbits. Thus, this method may be more effective than other methods without membrane anesthetic surgeries.
doi:10.15419/bmrat.v4i12.396 fatcat:6thzw2qj6nccli7nok5hbuqgqi