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The present study was carried out to investigate the effect of various refining processes' conditions on conjugated dienes in soybean oil. Conjugated dienes contents of soybean oil were found to undergo reduction in neutralization and bleaching steps while being increased in deodorization. It was observed that alkali refining (neutralization) through 12 and 14 °Bé, bleaching with either acid activated clay or a mixture of acid activated clay plus activated carbon at 110˚C along withfatcat:7425yq5uzzfo5b3dwkng6ots2e