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Key enzyme gene transformation is an ordinary and effective method to increase secondary metabolites of plants and microorganisms. In this work, a fungus expression vector (pV2 + -TS-pAN7-1) containing taxadiene synthase gene (ts), a rate-limiting enzyme gene for taxol biosynthesis, was constructed and used in the transformation of taxol-producing endophytic fungus EFY-21. Southern blot analysis demonstrated the integration of the ts gene in the genome of independent transformants and onedoi:10.5897/ajb10.1896 fatcat:ooy5n6nkgncg7dzscmnq6cxpma