Cooperation of protease-activated receptor 1 and integrin ανβ5 in thrombin-mediated lung cancer cell invasion

LINGYUN ZHU, XIUDONG WANG, JIE WU, DEKUI MAO, ZHISHAN XU, ZIYANG HE, AIPING YU
2012 Oncology Reports  
Protease-activated receptor 1 (PAR1) and integrins play an important role in thrombin-mediated tumor cell invasion. However, the role of PAR1 and integrin α ν β 5 and the relationship between the two receptors in thrombin-induced lung cancer invasion remains unknown. Moreover, the mechanisms through which immobilized thrombin facilitates tumor invasion are poorly understood. In this study, both native and immobilized thrombin promoted lung cancer cell adhesion, migration and extracellular
more » ... extracellular signal-regulated kinase phosphorylation. Integrin α ν β 5 is involved in both native and immobilized thrombin-mediated tumor cell invasion; PAR1 had no effect on immobilized thrombin-mediated cell invasion. PAR1 and integrin α ν β 5 colocalized on the surface of native thrombin-treated cells. This study suggests that targeting of integrin α ν β 5 or the PAR1-integrin α ν β 5 complex may present an important therapeutic opportunity to prevent lung cancer invasion.
doi:10.3892/or.2012.1851 pmid:22665038 fatcat:cdndzneuqfewvkupkseek4cmum