We investigated the effect of time, temperature, and the presence of sodium chloride, nitrates, and nitrites in the medium on the growth and production of enterotoxin B by Staphylococcus aureus. Assays by the double gel-diffusion method showed that m enterotoxin B production occurs at the beginning of the stationary phase of growth. Lowering the tanperature of incubation decreased the amount of toxin produced without affecting the total amount of growth. Increases in concentration of curing

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... s reduced toxin production more rapidly than cell growth. The relationship of these observations to food-poisoning outbreaks is briefly discussed. on May 4, 2020 by guest