ApiEST-DB: analyzing clustered EST data of the apicomplexan parasites

L. Li
2004 Nucleic Acids Research  
ApiEST-DB (http://www.cbil.upenn.edu/paradbsservlet/) provides integrated access to publicly available EST data from protozoan parasites in the phylum Apicomplexa. The database currently incorporates a total of nearly 100 000 ESTs from several parasite species of clinical and/or veterinary interest, including Eimeria tenella, Neospora caninum, Plasmodium falciparum, Sarcocystis neurona and Toxoplasma gondii. To facilitate analysis of these data, EST sequences were clustered and assembled to
more » ... consensus sequences for each organism, and these assemblies were then subjected to automated annotation via similarity searches against protein and domain databases. The underlying relational database infrastructure, Genomics Uni®ed Schema (GUS), enables complex biologically based queries, facilitating validation of gene models, identi®cation of alternative splicing, detection of single nucleotide polymorphisms, iden-ti®cation of stage-speci®c genes and recognition of phylogenetically conserved and phylogenetically restricted sequences.
doi:10.1093/nar/gkh112 pmid:14681425 pmcid:PMC308846 fatcat:7ovk5palcnaxtemxntecadggqq