Fluorescein-polyethyleneimine coated gadolinium oxide nanoparticles as T1 magnetic resonance imaging (MRI)–cell labeling (CL) dual agents

Wenlong Xu, Ja Young Park, Krishna Kattel, Md. Wasi Ahmad, Badrul Alam Bony, Woo Choul Heo, Seonguk Jin, Jang Woo Park, Yongmin Chang, Tae Jeong Kim, Ji Ae Park, Ji Yeon Do (+2 others)
2012 RSC Advances  
We report the synthesis, characterization and application of highly water-soluble fluoresceinpolyethyleneimine (PEI) coated gadolinium oxide (Gd 2 O 3 ) nanoparticles to magnetic resonance imaging (MRI) and cell labeling (CL). The average particle diameter and average hydrodynamic diameter were estimated to be 3.92 and 7.5 nm, respectively. Fluorescein-PEI was prepared from EDC/NHS coupling method. The surface coating was characterized by the FT-IR absorption spectrum and the surface coating
more » ... unt was estimated to be 22.42 wt% from a TGA analysis, corresponding to 0.65 nm 22 grafting density. The fluorescein-PEI coated gadolinium oxide nanoparticles showed r 1 and r 2 of 6.76 and 20.27 s 21 mM 21 , respectively, and a strong fluorescence at y527 nm. A pronounced positive contrast enhancement was clearly observed in 3 tesla T 1 MR images of a rat with a liver tumor after injection of an aqueous sample solution into a rat tail vein. After treatment of DU145 cells with a sample solution, a strong fluorescence in confocal images was also observed. These two results together confirm the excellent MRI-CL dual functionality of fluorescein-PEI coated gadolinium oxide nanoparticles. { Electronic supplementary information (ESI) available: XRD patterns of both as-prepared and TGA analyzed powder samples of fluorescein-PEI coated gadolinium oxide nanoparticles, a HRTEM image of the TGA analyzed powder sample, and NMR spectra. See
doi:10.1039/c2ra21052e fatcat:m3nstmy6gfaynig2rktkuuomey