Use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of Campylobacter jejuni and Campylobacter coli

D M Olive, M Johny, S K Sethi
1990 Journal of Clinical Microbiology  
A commercially available synthetic nucleic acid probe (SNAP) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting Campylobacter species. The SNAP was able to detect either 5 ng of C. jejuni DNA or 105 CFU of bacteria. The SNAP could also detect DNA extracted from 105 CFU
doi:10.1128/jcm.28.7.1565-1569.1990 fatcat:t5njbdxvvjc3fmnumdbqfhmrf4