VERVE Net Huttenhower Lab This tutorial focuses on performing a comprehensive metagenomic analysis from whole-genome shotgun sequencing data for microbiome studies. It is divided in to different steps that use the following metagenomic, computational tools: Me ta Phl An Me ta Phl An , G ra Phl An G ra Phl An , L EfSe L EfSe , and HUMAnN HUMAnN . Our last addition to the PhlAn tools is Phyl oPhl An Phyl oPhl An that can be used in metagenomics for phylgenetically and taxonomically place contigs

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... ssembled from whole metagenomic sequencing samples.This tutorial is also a step-by-step description of the metagenomic pipeline we used in our review paper about computational meta'omics (specifically Figure 4) .