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Hybrid error correction and de novo assembly of single-molecule sequencing reads
2012
Nature Biotechnology
Single-molecule sequencing instruments can generate multikilobase sequences with the potential to greatly improve genome and transcriptome assembly. However, the error rates of single-molecule reads are high, which has limited their use thus far to resequencing bacteria. To address this limitation, we introduce a correction algorithm and assembly strategy that uses short, highfidelity sequences to correct the error in single-molecule sequences. We demonstrate the utility of this approach on
doi:10.1038/nbt.2280
pmid:22750884
pmcid:PMC3707490
fatcat:wiou4fgizjdobow5mbedgzyqm4