Noninvasive harmonics optical microscopy for long-term observation of embryonic nervous system development in vivo

Szu-Yu Chen, Cho-Shuen Hsieh, Shi-Wei Chu, Cheng-Yung Lin, Ching-Yi Ko, Yi-Chung Chen, Huai-Jen Tsai, Chin-Hwa Hu, Chi-Kuang Sun
<span title="">2006</span> <i title="SPIE-Intl Soc Optical Eng"> <a target="_blank" rel="noopener" href="" style="color: black;">Journal of Biomedical Optics</a> </i> &nbsp;
Nervous system development is a complicated dynamic process, and many mechanisms remain unknown. By utilizing endogenous second-harmonic-generation as the contrast of polarized nerve fibers and third-harmonic-generation ͑THG͒ to reveal morphological changes, we have successfully observed the vertebrate embryonic nervous development from the very beginning based on a 1230-nm light source. The dynamic development of the nerve system within a live zebrafish embryo can be recorded continuously more
more &raquo; ... than 20 hr without fluorescence markers. Since the THG process is not limited by the time of gene expression and differentiation as fluorescence-based techniques are, the observable stages can be advanced to the very beginning of the development process. The complete threedimensional brain development from a neural plate to a neural tube can be uncovered with a submicron lateral resolution. We have, for the first time, also reported the generation of SHG from myelinated nerve fibers and the outer segment of the photoreceptors with a stacked membrane structure. Our study clearly indicates the fact that higher-harmonics-based optical microscopy has the strong potential to long-term in vivo study of the nervous system, including genetic disorders of the nervous system, axon pathfinding, neural regeneration, neural repair, and neural stem cell development.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="">doi:10.1117/1.2363369</a> <a target="_blank" rel="external noopener" href="">pmid:17092171</a> <a target="_blank" rel="external noopener" href="">fatcat:5yhfzqwdt5ep7nkcho24owwzwq</a> </span>
<a target="_blank" rel="noopener" href="" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href=""> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> Publisher / </button> </a>