CHANGES IN PGE2 AND TXB2 FORMATION BY PLATELETS OF ATHEROSCLEROTIC RABBITS

G Laekeman, A Van Hoydonck, M Van Diest, A Herman
1987 SUPPLEMENTARY ABSTRACTS   unpublished
The synthesis of thromboxane B2 (TXB2) and prostaglandin E2 (PGE2) were studied in groups of 9 rabbits fed a normal diet or a diet containing 0.3 % of cholesterol. After 16 weeks, a platelet rich suspension containing 300.000 platelets/mm3 was prepared. Portions were incubated at 37°C with arachidonic acid (AA). Biosynthesis of PGE2, TXA2 and TXB2 was evaluated by the bioassay cascade system (rabbit coeliac and mesenteric artery and rat fundus strip) and by radioimmunoassay (RIA) :No
more » ... RIA) :No significant differences were recorded for TXB2 after 1 or 10 minutes incubationA small portion of blood without EDTA was allowed to clot at 37°C during 1 hour. TXB2 was measured in the serum by RIA. The mean ± SEM obtained for the atherosclerotic rabbits (82.70 ± 9.28 ng/ml) was significantly higher than the one for the normal group (42.66 ± 11.45 ng/ml) (P < 0.02, n = 9 for both groups. From these results it can be concluded that platelets of atherosclerotic rabbits apparently synthetize less PGE2 when incubated with AA, and more TXB2 during a standardized clotting process
doi:10.1055/s-0038-1644812 fatcat:w2eef2vsqvg7nkm7vj4zsj4nge