Sensitive determination of amlodipine besylate using bare/unmodified and DNA-modified screen-printed electrodes in tablets and biological fluids

Mohamed Khairy, Ahmed A. Khorshed, Farouk A. Rashwan, Gamal A. Salah, Hanaa M. Abdel-Wadood, Craig E. Banks
2017 Sensors and actuators. B, Chemical  
The screen-printed technique is widely used as an efficient tool for electrochemical analysis in environment, clinical and agri-food areas. Significantly, it has the ability to transfer electrochemical laboratory experiments into the field. In the present work, we report a highly sensitive, simple, low-cost protocol for determination of amlodipine (AML) using bare/unmodified and DNA-modified screen-printed electrodes (SPEs). The immobilization of DNA molecules onto SPE offers promising robust
more » ... d chemically stable molecular wires, which provides a unique opportunity for charge transfer processes. Consequently, the electroanalytical sensing of AML was explored at bare/unmodified and DNA-modified SPEs in a linear range between 0.066-1.0 M and 0.066-2.0 M with the detection limit (3) found to be 20.70 nM and 14.94 nM, whilst corresponding sensitivities of: 0.43 A L mol −1 and 4.23 A L mol −1 respectively. Although, the superior electrochemical signature of bare SPEs is evident, the immobilization of DNA onto SPEs enhances the sensitivity 10-times more than the bare SPEs. Furthermore, the optimized electroanalytical protocol using the unmodified SPEs, which requires no pre-treatment and electrode modification step, was then further applied to the determination of AML in real samples. Crown . His current research interests are control synthesis of porous metal oxide nanostructures for developing electrochemical sensors.
doi:10.1016/j.snb.2016.07.165 fatcat:wp55ndebonfahc6hmkiwya23ri