Recently, advance bio-sensing devices have given considerable importance, because of their capability to identify the target compounds promptly. In this context,2 nd generation glucose biosensor has been fabricated using natural redox compound, 5-methyl 1, hydroxyphenazine (5-MHP)obtained from P. aeruginosa. Amperometric detection was based on interaction of glucose with the working electrode loaded with activated carbon (AC), 5-MHP and glucose oxidase (GOx), AC/5-MHP/GOx. Working electrodes

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... pared at three different temperatures 4 o C, 25 o C and 47 o C, sensitively detect glucose andshowed the linear ranges of R 2 = 0.98, R 2 = 0.98 and R 2 = 0.99 respectively with detection limit of 0.3µM at signal-to-noise ratio (S/N)=3. It was found that GOx immobilization temperature directly influence the long term efficiency of glucose biosensor. Electrode fabricated at 4 o C exhibited greater operational stability i.e. 74% followed by 25 o C (68%) and 47 o C (48%). Furthermore, theresponse timewith eachglucoseconcentration (2.0 to 26.0mM) was relatively less i.e. 2s, for enzyme electrode fabricated at 4 o C whereas it was 4s and 5s for working electrodes prepared at 25 o C and 47 o C respectively.