Visible Study of Mercuric Ion in Brassica Juncea Living Cells and Plants Using a Fluorescent Dye

Pin-san XU, Zheng-yao ZHANG, Ling-yao LI, Yu-bo LIU
2017 Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)   unpublished
Concern over mercury cytotoxicity inside live specimens has encouraged the development of efficient methods for the in vivo detection of this heavy metal. In this study, by using the previous developed fluorescence dye EPNP (2,6-bis-(4'-peperazino-N'-butyl-1',8'-naphthalimide) dimethylpyridine) as a highly selective and sensitive probe for mercuric ions (Hg 2+ ), the images of Hg 2+ distribution and transportation in the living cells and plants of Brassica juncea were obtained. After 10, 50,
more » ... d. After 10, 50, 100 μM Hg 2+ exposure, gradually increased fluorescence was detected in B. juncea living cells loaded with EPNP dye. Intracellular Hg 2+ time-dependent fluorescence enhancement of EPNP was also observed in B. juncea cells. Furthermore, images of confocal microscope shown that Hg 2+ was found to mostly accumulate in lytic vacuole but not in the nucleus or mitochondria. In addition, a concentration-dependent rise of fluorescence was observed when the EPNP interacted with an increasing concentration of Hg 2+ in the plants of B. juncea. These results provided direct experimental evidences for the distribution, translocation and transportation of Hg 2+ in B. juncea. EPNP would be used as a chemical tool for the study of phytoremediation related researches.
doi:10.2991/bbe-17.2017.18 fatcat:7yih5wwzcfh5xkgyxmlhc7b4ze