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Comparative evaluation of cDNA library construction approaches for RNA-Seq analysis from low RNA-content human specimens
2018
Journal of Microbiological Methods
With the emergence of RNA sequencing technologies, metatranscriptomic studies are rapidly gaining attention as they simultaneously provide insight into gene expression profiles and therefore disease association pathways of microbial pathogens and their hosts. This approach, therefore, holds promise for applicability in infectious disease diagnostics. A challenge of this approach in the clinical setting is the low amount and quality of RNA, especially microbial RNA in most clinically-infected
doi:10.1016/j.mimet.2018.10.008
pmid:30332617
pmcid:PMC6242773
fatcat:odtd653vbbfafc6srgcr6kdipi