Lowered insulin-sensitive Ca2+ transport in cultured glomerular mesangial cells from spontaneously hypertensive rats

SATORU KURIYAMA, HARUO TOMONARI, HIROSHI SEGAWA, MAKOTO HORIGUCHI, TAKAO HASHIMOTO, OSAMU SAKAI
1993 The Japanese Journal of Nephrology  
Insulin-resistant state has been postulated in the pathogenesis of hypertension. To investigate the role of insulin in Ca2+ regulation of the glomerular mesangial cell (MC) in hypertension, the effect of insulin on Ca2 transport and intracellular-free calcium concentration ([Ca2+],) was measured in serially passaged cultured MC obtained from spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Insulin produced a substantial increase in 45Ca uptake as well as [Ca2+] ; in quiescent
more » ... ultured MC from both strains. These stimulatory effects of insulin were completely inhibited by diltiazem, and partially inhibited by H-7, TMB-8 and 5-N,N (hexamethylene) amiloride (HMA), but were not inhibited by W-7 or trifluoroperazine. Although basal ([Ca2+];) values were not different, insulin-sensitive 45Ca uptake of SHR MC was significantly less than that of WKY MC. Insulin-sensitive increase in ([Ca2+];) of SHR MC was also less than that of WKY MC. We concluded' that insulin increased 45Ca uptake, leading to an increase in ([Ca2+];), presumably through the voltage-dependent Ca2+ channel, intracellular Ca2+ release and/or proteinkinase C-mediated mechanisms in cultured MC. Blunted response of insulin-sensitive Ca2 uptake and ([Ca2+];) in SHR MC suggested differential regulation of Ca2+ transport in response to insulin in the kidney with primary hypertension.
doi:10.14842/jpnjnephrol1959.35.893 fatcat:5p7jcukj4bbcrjdrqnsfnxrgau