Cytoplasmic IRE1α-mediatedXBP1mRNA Splicing in the Absence of Nuclear Processing and Endoplasmic Reticulum Stress

Sung Hoon Back, Kyungho Lee, Elizabeth Vink, Randal J. Kaufman
2006 Journal of Biological Chemistry  
3 The abbreviations used are: ER, endoplasmic reticulum; UPR, unfolded protein response; EGFP, enhanced green fluorescence protein; XBP1, X-box binding protein 1; EMCV, encephalomyocarditis virus; IRES, internal ribosomal entry site; DHFR, dihydrofolate reductase; CHO, Chinese hamster ovary; Tm, tunicamycin; Tg, thapsigargin; Act D, actinomycin D; DRB, 5,6-dichloro-1-␤-D-ribofuranosylbenzimidazole; nt, nucleotide(s); MEF, mouse embryonic fibroblast; RT, reverse transcriptase; PBS,
more » ... PBS, phosphatebuffered saline; eIF, eukaryotic initiation factor; HA, hemagglutinin; TRITC, tetramethylrhodamine isothiocyanate; PERK, PKR-related endoplasmic reticulum eukaryotic initiation factor 2␣ kinase.
doi:10.1074/jbc.m602030200 pmid:16644724 fatcat:kkwcv3pi4ng4ll3r265nqqec7i