Isolating and Identifying Organic Acids from Portulaca oleracea and Determining Their Anti-cyanobacterial Activity

Hongqiang Wang, Lieyu Zhang, Yangyang Wang
2017 Polish Journal of Environmental Studies  
Introduction Microcystis aeruginosa is a common freshwater cyanobacterium in freshwater lakes and reservoirs worldwide. Microcystins produced by M. aeruginosa have been implicated in wildlife, livestock, and pet fatalities as well as human poisonings worldwide [1] [2] [3] [4] [5] [6] [7] . The control of Microcystis blooms is an urgent issue with regard to improving water and ecosystem quality and public health. In recent efforts to control toxic bloom-forming Microcystis, algicides from
more » ... gicides from natural biomaterials (allelochemicals) have received attention as alternatives to chemical agents [8] . Traditional approaches to studying allelochemicals have focused on the isolation of allelopathic compounds from aquatic macrophytes [9] [10] [11] [12] . A few studies have focused on the potential algaecide isolation from terrestrial plants, such as barley straw, Salvia miltiorrhiza Bung, and Radix Astragali [13] [14] [15] [16] . Abstract We explored the possibility of using allelochemicals from terrestrial plants to solve the problem of cyanobacterial blooms in eutrophicated shallow lakes. The highly effective inhibitory allelochemicals of purslane (Portulaca oleracea L.) and purslane seeds were extracted and purified through a series of procedures, and the chemical components of the purified extracts were analyzed by gas chromatographymass spectrometry (GC-MS). The extracts contained 12 fatty acids and three phenolic acids, of which nine compounds were found to be inhibitors of harmful cyanobacteria. The inhibitory ratios of the purified extracts of purslane and purslane seeds on M. aeruginosa were 97.4 and 81.6%, respectively, when the cyanobacterial were exposed under 15.0 mg/L of extracts. The purified extracts exhibited significant anticyanobacterial activities, the organic acids that may contribute to the allelopathic effects of the purslane and purslane seeds on M. aeruginosa.
doi:10.15244/pjoes/64465 fatcat:3g7sprvenrejrazrvmkuxndjhi