ORIGINAL ARTICLES In vitro Propagation and Somatic Embryogenesis in Egyptian Husk Tomato (Physalis pubescens L.)

Mona Yousry
2013 Journal of Applied Sciences Research   unpublished
Efficient and reproducible methods for micropropagation and somatic embryogenesis of Egyptian husk tomato Balady species (physalis pubescens L.), that belongs to family Solanaceae, were tested. Seedlings of tested cultivar were germinated which cultured on hormone free-medium then shoot tip explants were transplanted onto MS medium for initiation supplemented with the cytokinin BAP at (0.000, 0.125, 0.250, 0.500 and 1.000 mg/l) in combinations with the auxin NAA (0.000, 0.125 and 0.250 mg/l).
more » ... and 0.250 mg/l). During the multiplication stage, the nodel cuttings of initiation stage were cultured on MS medium supplemented with cytokinin BAP at (0.000, 0.250, 0.500, 1.000 and 2.000 mg/l) in combinations with the auxin NAA (0.000, 0.125 and 0.250 mg/l). For the rhizogenesis stage, the explants derived from the multiplication stage were cultured onto MS medium supplemented with the NAA at different concentrations (0.000, 0.125, 0.250, 0.500, 1.000 and 2.000). The rametes rooted shoots were transferred ex vitro and in vivo under shading and then acclimatized, gradually. The acclimatized neoformed plantlets were established and appeared healthy and true-to-type. Asexual embryos were arose from embryogenic callus derived from leaf explants when cultured on MS medium augmented with 2,4-D at (0.000, 0.500, 1.000, 2.000 and 4.000 mg/l) in combinations with BAP at (0.00, 0.125 and 0.250 mg/l), and somatic embryos were obtained as another method for in vitro vegetative propagation.