Identification and characterization of polymorphisms at the HSA α1-acid glycoprotein (ORM*) gene locus in Caucasians

Catherine M. Owczarek, Aleksander L. Owczarek, Philip G. Board
2002 Genetics and Molecular Research  
Human α 1 -acid glycoprotein (AGP) or orosomucoid (ORM) is a major acute phase protein that is thought to play a crucial role in maintaining homeostasis. Human AGP is the product of a cluster of at least two adjacent genes located on HSA chromosome 9. Using a range of restriction endonucleases we have investigated DNA variation at the locus encoding the AGP genes in a group of healthy Caucasians. Polymorphisms were identified using BamHI, EcoRI, BglII, PvuII, HindIII, TaqI and MspI. Nonrandom
more » ... sociations were found between the BamHI, EcoRI and BglII RFLPs. The RFLPs detected with PvuII, TaqI and MspI were all located in exon 6 of both AGP genes. The duplication of an AGP gene was observed in 11% of the individuals studied and was in linkage disequilibrium with the TaqI RFLP. The identification and characterization of these polymorphisms should prove useful for other population and forensic studies.
doi:10.4238/2002.march.30.10 fatcat:fw7t2mufhzehzpcqa5kfzdcvuu