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In vitro immunological activities of the polysaccharide fraction from Haemophilus influenzae type a endotoxin
1982
Infection and Immunity
Mild hydrolysis of Haemophilus influenzae type a lipopolysaccharide by ion exchangers in the presence of chloroform, to remove the lipid moiety, yelded a nontoxic and immunogenic polysaccharide fraction. This polysaccharide selectively triggered murine B lymphocytes in vitro: (i) it induced enhancement of thymidine incorporation and stimulated antibody secretion in cultures of normal and nude mouse spleen cells; (ii) it did not stimulate splenic T lymphocytes; (iii) the activation of B
doi:10.1128/iai.36.2.603-608.1982
fatcat:k2b5qrf4e5bxdnr44iprf4prge