Characterization of Rapidly Degraded Polypeptides in Mammalian Cells Reveals a Novel Layer of Nascent Protein Quality Control

Shu-Bing Qian, Michael F. Princiotta, Jack R. Bennink, Jonathan W. Yewdell
2005 Journal of Biological Chemistry  
FIGURE 4. Ubiquitin-independent degradation of a defined DRiP substrate. A, E36 and ts20 cells were preincubated at 32 or 41°C for 2 h and then infected with a rVV-expressing minigene MSIINFEKL at 37°C for the times indicated. K b -SIINFEKL complex formation was determined by flow cytometry using Alexa 647-conjugated 25-D1.16 monoclonal antibody. Note that E36 cells express greater amounts of K b than ts20 cells, and the y -axes in this panel (and panels B and C) have been normalized to account
more » ... for this difference. B and C, E36 cells (upper panel) and ts20 cells (bottom panel) were preincubated at 32 or 41°C for 2 h and then infected with rVV expressing (R)NP-S-GFP (B) or NP-S-GFP (C) at 37°C for the times indicated. GFP fluorescence and K b -SIINFEKL complex formation were determined by flow cytometry.
doi:10.1074/jbc.m509126200 pmid:16263705 fatcat:4fkro6jd2zhmzhadiqmuqtkhl4