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An mRNA differential display strategy for cloning genes expressed during mouse gonad development
International Journal of Developmental Biology
The mRNA differential display technique has become a popular method for isolating novel genes in a variety of biological systems including carcinogenesis, hormone regulation, plant biology and neurobiology. We have further developed the method by optimizing different steps for the use of small amounts of material, such that differential display can be used in the study of developmental biology. Our techniques include a new assay for elimination of false positive cDNA clones and apmid:9303351 fatcat:u6lvpw6hv5ezjpjy2qonx2gzge