Neurohypophysial hormone receptors and second messengers in trout hepatocytes
Journal of Endocrinology
Neurohypophysial hormone receptors and second messengers were studied in trout (Oncorhynchus mykiss) hepatocytes. Arginine vasotocin (AVT) and isotocin (IT) elicited a concentration-dependent inhibition of cAMP accumulation in the presence of 5 10 -8 M glucagon (maximal effect for 4·5 10 -7 M and 1·4 10 -7 M, half-maximal effect for 2·1 10 -8 M and 0·7 10 -8 M, AVT and IT respectively). The effect of glucagon was inhibited up to 90% by AVT and 80% by IT. While AVT inhibited (up to 50%) the
... up to 50%) the basal cAMP production, IT had no such action. Specific V 1 or V 2 analogues (with reference to vasopressin in mammals) were used for pharmacological characterization of the type of neurohypophysial hormone receptor involved in this inhibition. The V 1 agonist [Phe 2 , Orn 8 ]-oxytocin inhibited the glucagon-stimulated cAMP production with a maximal effect for 6 10 -7 M and a half-maximal effect for 0·9 10 -8 M concentrations of the analogue. While the V 1 agonist reduced the glucagonstimulated cAMP level by 70%, it showed only a tendency to reduce the basal level. The V 2 agonist [deamino 1 , Val 4 , -Arg 8 ]-vasopressin had no effect either on basal or on glucagon-stimulated cAMP production. The V 1 antagonist [d(CH 2 ) 5 1 , O-Me-Tyr 2 , Arg 8 ]-vasopressin totally reversed the 10 -8 M AVT-induced inhibition of 5 10 -8 M glucagon-stimulated cAMP production, whereas the V 2 antagonist [d(CH 2 ) 5 1 , -Ile 2 , Ile 4 , Arg 8 , Ala 9 ]-vasopressin had no such effect. In this particular case, maximal and half-maximal effects of the V 1 antagonist were obtained for 2·3 10 -6 M and 1·2 10 -6 M respectively. Changes in intracellular calcium content were measured using the fluorescent probe FURA-2/AM. AVT and IT elicited a concentration-dependent increase in Ca 2+ accumulation. The comparison of the effect of 10 -8 M agonists versus AVT showed the following order of potency: AVT=IT>V 1 agonist>V 2 agonist. The V 1 antagonist reversed the AVT-induced Ca 2+ accumulation whereas the V 2 antagonist had no such effect. These results are taken as evidence for the presence in trout hepatocytes of neurohypophysial hormone receptors functionally close to the V 1a -type linked to cAMP production and Ca 2+ mobilization.