Quantifying cell-adhesion strength with micropipette manipulation: principle and application

Jin-Yu Shao
2004 Frontiers in Bioscience  
TABLE OF CONTENTS 1. Abstract 2. Introduction 3. The step-pressure technique 4. The biomembrane-force probe 5. The micropipette-aspiration technique 6. Requirements of the MAT 7. Applications of the MAT 8. Concluding remarks 9. Acknowledgements 10. References ABSTRACT Quantifying cell-adhesion strength is of great importance in biology and medicine. Cell-adhesion strength can be characterized by separating two adherent cells and determining the force required to do so, or by measuring the
more » ... measuring the lifetime of a receptor-ligand bond that mediates cell adhesion. To this end, several micropipette-based experimental techniques that operate at both cellular and molecular levels have been developed over the past few decades. In this review, we provide an overview of three of these techniques, i.e., the step-pressure technique (SPT), the biomembraneforce probe (BFP), and the micropipette-aspiration technique (MAT). More detailed discussion will be given about the requirements and applications of the MAT.
doi:10.2741/1386 pmid:15353280 fatcat:vt2juxauijbqxaftfybazutpoy