Improvement of a miRNA inhibitor by intracellular selection

Akira Tachibana, Aiko Yamamoto
2020 Figshare  
Sequences surrounding the miRNA binding domain of the miRNA inhibitor LidNA were selected intracellularly. The library was transfected into cells, and then, inhibitors that were associated with argonaute 2 were selected. The potent inhibitors were slowly degraded intracellularly, while the lower-activity inhibitors were rapidly degraded. A combination of the selected sequences surrounding the miRNA binding domain enhanced miRNA inhibitory activity. Abbreviations: LidNA: DNA that puts a lid on
more » ... hat puts a lid on miRNA function; LNA: locked nucleic acid; Ago2: argonaute 2; LNA: locked nucleic acid
doi:10.6084/m9.figshare.11992965 fatcat:tjswgxltg5gwfhu42bp5apazzq