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Mouse R1-syntrophin sequences were produced as chimeric fusion proteins in bacteria and found to bind phosphatidylinositol 4,5-bisphosphate (PtdIns4,5P 2 ). Half-maximal binding occurred at 1.9 µM PtdIns4,5P 2 and when 1.2 PtdIns4,5P 2 were added per syntrophin. Binding was specific for PtdIns4,5P 2 and did not occur with six other tested lipids including the similar phosphatidylinositol 4-phosphate. Binding was localized to the N-terminal pleckstrin homology domain (PH1); the second,doi:10.1021/bi982564+ pmid:10220348 fatcat:uadpc4if5bb75hsg2cbjrbqj24