In this study, gold nanoparticles and thermochromic composite films modified screen-printed carbon electrodes (TM-AuNPsSPCEs) were developed as a platform for the simultaneous detection of protein and temperature. The TM-AuNPs composited film had better sensitivity resulting from the gold nanoparticles amplification effect. A phase transition model analysis of TM-AuNPs films found that the TM-AuNPs films had three-phase transition intervals (<45˚C, 45˚C to 80˚C and >80˚C) which accommodated the

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... temperature requirements for protein denaturation. When used to detect different concentrations of haemoglobin (Hb) solution, the TM-AuNPs modified SPCEs had a better sensitivity in detecting the different concentrations in comparison to TM and AuNP modified SPCEs which showed no clear sensitivity towards the different Hb concentrations. The dual detection and excellent sensitivity show a good application prospect for the study of the TM-AuNPs composite film. protein biosensors [2] [3] [4]. Proteins exist in all organisms and are very important biological macromolecules. It is the executor of biological functions and plays a variety of physiological functions such as biocatalysis, material transport, movement, defense, regulation, memory, and recognition [5] [6] [7] . A very important condition for protein to realize these functions is to keep the environment at a certain temperature. With a change in temperature, some functions cannot be achieved because of changes in protein content and structure. In the last two decades, the research on the effect of temperature on protein mainly fo-