Internet Archive Scholar

Identification of the Arabidopsis thaliana Flavonoid 3'-Hydroxylase Gene and Functional Expression of the Encoded P450 Enzyme

C. Schoenbohm, S. Martens, C. Eder, G. Forkmann, B. Weisshaar
2000 Biological chemistry  

Preserved Fulltext

fulltext thumbnail
Web Archive Capture PDF (297.4 kB)
https://web.archive.org/web/20170812132643/http://www.floramare.de/content/Schoenbohm%20et%20al.%20Biol.%20Chem.%202000%20Arabidopsis%20F3%27H%20expression.pdf

A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2017; you can also visit the original URL. The file type is application/pdf.

The phenylpropanoid pathway results in the synthesis of thousands of compounds, including flavonoids like flavonols, anthocyanidins and tannins. In Arabidopsis thaliana, the lack of tannins in the seed coat (testa) causes the transparent testa (tt) phenotype. In the present study, we identified the gene responsible for the tt7 mutation. We show that TT7 encodes the enzyme flavonoid 3'-hydroxylase (F3'H), and demonstrate that this P450-dependent monooxygenase has F3'H activity. The availability
more » ... f the AtF3'H gene and promoter sequence will allow us to study the coregulation of a complete set of flavonol and anthocyanidin biosynthesis genes in A. thaliana, and makes in vitro synthesis of hydroxylated flavonoids more feasible.
doi:10.1515/bc.2000.095 pmid:11030432 fatcat:2dx7lmnksbdedmerjcc36frjym
Citation

C. Schoenbohm, S. Martens, C. Eder, G. Forkmann, B. Weisshaar. "Identification of the Arabidopsis thaliana Flavonoid 3'-Hydroxylase Gene and Functional Expression of the Encoded P450 Enzyme." Biological chemistry 381.8 (2000)