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Structural and antigenic conservation of the P2 porin protein among strains of Haemophilus influenzae type b
Infection and Immunity
The P2 porin protein is the most abundant protein in the outer membrane of Haemophilus influenzae type b (Hib). Biochemical and immunochemical techniques were used to characterize the P2 proteins from a number of different Hib strains. P2 proteins from Hib outer membrane vesicles were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose for in situ tryptic digestion. Solid-phase tryptic digests of P2 from eight Hib strains were resolved bydoi:10.1128/iai.57.11.3270-3275.1989 fatcat:ypji3tfssnc7vpaz7g3lubp4pe