Sertoli cells as paracrine modulators of DNA synthesis in rat peritubular myoid cells in culture

S. S. Raychoudhury, E. W. Thompson, A. W. Blackshaw, M. G. Irving
1993 Reproduction  
H]thymidine incorporation by peritubular myoid cells. This indicates a cell\p=n-\cell cooperation between Sertoli and peritubular myoid cells in the testis in terms of DNA synthesis. Secreted factors from Sertoli cells, as tested in a parabiotic culture situation, also increased [3H]thymidine incorporation by peritubular myoid cells. Moreover, in terms of total cellular protein, cocultures of Sertoli cells and peritubular myoid cells resulted in a significant increase when compared with the
more » ... mpared with the monocultures, and this coculture effect substituted for the stimulatory response of serum on peritubular myoid cell monoculture. This study investigated the cooperative role of Sertoli cells and peritubular myoid cells in paracrine regulation of testicular functions. (FCS) were supplied by Gibco Laboratories (Grand Island, NY).
doi:10.1530/jrf.0.0990513 pmid:8107034 fatcat:gc2znwpjjrbojj5adjs6hsbv2e