Diagnosis of Bacterial Vaginosis among Symptomatic Egyptian Women: Value of Assessing Bacterial Sialidase Activity
International Journal of Current Microbiology and Applied Sciences
The development of point-of-care tests that are accurate, simple, rapid, low cost, and that do not require high levels of training for their interpretation is integral to improving the syndromic management algorithms for vaginal discharge that at present perform poorly. The present study aimed at evaluating the usefulness of assessing the bacterial sialidase activity as a rapid, sensitive and specific alternative compared to routine diagnostic methods for diagnosis of bacterial vaginosis (BV).
... al vaginosis (BV). The study included 50 female patients presenting with excessive and/or abnormal vaginal discharge. Four swabs were used to collect the vaginal discharge from each patient and were examined by culture, Amsel's criteria, Gram stain using the Nugent's score and BVBlue test to assess the sialidase activity. BV was detected in 18% of the studied women by all of the diagnostic methods. Compared to Nugent's score ≥7, the detection of sialidase by BVBlue test had 100% sensitivity and 84.6% specificity. There was a good agreement between the results of BVBlue test and clinical diagnosis using Amsel's criteria. We concluded that the determination of sialidase activity by BVBlue test can be a simple, rapid, and sensitive alternative to routine diagnostic methods that require expert manpower and sophisticated techniques.