Many studies done in past literature focuses on gonadal toxicity of Cyclophosphamide, but very less studies are done on the epididymis post exposure to this drug. Hence a sincere attempt is made in present study to look into the cellular changes in the epididymis through histology and TUNEL Assay. Subjects & Methods: Healthy male Swiss albino adult mice, were obtained from Central Animal Research Facility, Manipal were used for the study. The adult as well as prepubertal male mice were divided

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... nto control and test groups. In test group, the mice were injected with Cyclophosphamide at variable doses. From each group certain no. of mice was sacrificed on different days. A control group was kept for each intervals. The epididymal tissue was then extracted and used in histological and Tunnel Assay study. Results: Even though there was no significant change in the number of basal cells, the apical cells decreased marginally at higher doses of CP. A TUNEL assay was performed in the epididymal sections to understand its effect on the DNA integrity. Up to 100 mg/kg, CP did not induce any significant increase in the TUNEL positive tubules. However, at 200 mg/kg dose and above, there was an increase in the number of tubules with TUNEL positive cells indicating its DNA damaging effect. However, this increase was statistically not significant Conclusion: The present study highlights the need for looking into the cellular damage of the cells after exposure to one of the commonly used anticancer drug- cyclophosphamide. Keywords: Epididymal Histology, Tunnel Assay, Cyclophosphamide, Swiss Albino mice.