An inhibitory compound produced byPseudomonaswith effectiveness onVibrio harveyi

Radhakrishnan Preetha, Seena Jose, Sreedharan Prathapan, Koyadan K Vijayan, Naatamai S Jayaprakash, Rosamma Philip, Isaac S Bright Singh
2009 Aquaculture Research  
Persistence of the antivibrio property of the potential antagonistic probiotics, Pseudomonas MCCB 102 and 103, at di¡erent temperatures, pH and in organic solvents was studied. The antivibrio compound was extracted, puri¢ed and characterized using thin-layer chromatography, high-pressure liquid chromatography, liquid chromatography-mass spectroscopy, UVV is and nuclear magnetic resonance spectroscopy and identi¢ed as N-methyl-1-hydroxyphenazine, a phenazine antibiotic. The toxicity of the
more » ... nd was tested in Penaeus monodon haemocyte culture and the IC 50 value was found to be1.4 AE 0.31mg L À 1 . The compound was found to be bacteriostatic at 0.5 mg L À 1 . Its stability to varying temperature, pH, organic solvents, prolonged shelf-life and vibriostatic nature point to its suitability for prophylatic aquaculture application. Solvent extraction was performed using 1-butanol, ethyl acetate, dichloromethane, chloroform and hex- Aquaculture Research, 2010 An inhibitory compound againstVibrio harveyi R Preetha et al.
doi:10.1111/j.1365-2109.2009.02436.x fatcat:an3yymlymfeqroqbjdvwhhbvdm