Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line
American Journal of Physiology - Cell Physiology
Bepoldin. Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line. Am J Physiol Cell Physiol 284: C1054-C1064, 2003 10.1152/ ajpcell.00446.2002.-Previously, we showed that the peak density of the transient outward K ϩ current (Ito) expressed in GH3 cells was different in the S phase than in other phases of the cell cycle. Using cell synchronization, we show here that I to drops precisely at the quiescent (G0 phase)/proliferating transition. This
... ng transition. This change is not due to a modification in the voltage dependence of I to, but rather to a modification in its inactivation kinetics. Molecular determination of K ϩ channel subunits showed that Ito required the expression of Kv1.4, Kv4.1, and Kv4.3. We found that the increase in I to density during the quiescent state was accompanied by an increase in Kv1.4 protein expression, whereas Kv4.3 expression remained unchanged. We further demonstrate that the link between I to expression and cell proliferation is not mediated by variations in cell excitability. These results provide new evidence for the cell cycle dependence of I to expression, which could be relevant in understanding the mechanisms leading to pituitary adenomas.