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The study of enzymes produced by thermophilic bacteria continues to grow. One of the enzymes produced by this bacteria is a protease enzyme which is very useful in various fields of life. This article will discuss how to identifying protease producing thermophilic bacteria with 16S rRNA gene analysis.doi:10.5281/zenodo.4892794 fatcat:dvytzvurhfhc7muahymzaijrny