ded in paraffin. Consequently, formalin crosslinking may Antigen-retrieval methods and their applications are ex-mask tissue antigens, thus preventing antibody access. tremely important for quality control and for the standard-Crosslinking properties can be reversed by placing tissues in ization of immunohistochemical techniques. Over the last different types of retrieval buffers and super-heated temdecade, many contributors, including industry, doctors, his-peratures between 80 and 125°C.