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OBJECTIVES: A fully automated on-line solid phase extraction procedure was developed and validated for the analysis of 30 key proteins biomarkers in Gammarus fossarum. METHODS: After protein extraction and tryptic digestion, peptides were cleanedup onto an on-line SPE cartridge (Oasis HLB, 2.1 mmx20 mm, 25 μm particle size) coupled to a LC-MS/MS system. The SRM assay was performed on a quadrupole-trap mass spectrometer. RESULTS: The method targeted 30 proteins in G. fossarum (46 reporterdoi:10.17145/jab.18.012 fatcat:dpu2afqabjaqbff5776dblqluu