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We used a high-resolution mid-IR tunable-laser absorption spectroscopy ͑TLAS͒ system with a single IV-VI laser operating near 5.2 m to measure the level of exhaled nitric oxide ͑eNO͒ in human breath. A method of internal calibration using simultaneous eNO and exhaled CO 2 measurements eliminated the need for system calibration with gas standards. The results observed from internally calibrating the instrument for eNO measurements were compared with measurements of eNO calibrated to gasdoi:10.1364/ao.41.006018 pmid:12371564 fatcat:irqczskkczd2vbn5v5ve7s7jge