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A new express method based on lateral flow immunoassay (LFIA) for progesterone detection was developed. To increase the assay sensitivity an enzyme label (horse radish peroxidase) was used instead of col loidal gold. An optimal assay format was chosen and the influence of a range of buffer supplements (deter gents, proteins and sucrose) was investigated by enzyme linked immunosorbent assay (ELISA). Linear range of LFIA was between 2 and 40 ng/mL in buffer. Limit of detection was 2 ng/mL, assay time was within 15 min.doi:10.3103/s0027131412050045 fatcat:73qf5oxqjvb35icjtj5pxifb4u